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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using JP45/JSRP1 Polyclonal antibody (10854-1-AP)

    WB analysis of human skeletal muscle using 10854-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10854-1-AP (JP45/JSRP1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using JP45/JSRP1 Polyclonal antibody (10854-1-AP)

    WB analysis of human skeletal muscle using 10854-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10854-1-AP (JP45/JSRP1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using JP45/JSRP1 Polyclonal antibody (10854-1-AP)

    IHC staining of human pancreas cancer using 10854-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10854-1-AP (JP45/JSRP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using JP45/JSRP1 Polyclonal antibody (10854-1-AP)

    IHC staining of human pancreas cancer using 10854-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10854-1-AP (JP45/JSRP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using JP45/JSRP1 Polyclonal antibody (10854-1-AP)

    IF Staining of HepG2 using 10854-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using JP45/JSRP1 antibody (10854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

JP45/JSRP1 Polyclonal antibody

JP45/JSRP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10854-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

JSRP1, Junctional-face membrane protein of 45 kDa homolog, JP-45, JP45, JP 45

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman skeletal muscle tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10854-1-AP targets JP45/JSRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1300

Product name: Recombinant human JP45; JSRP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-331 aa of BC021201

Sequence: MSMTTRAWEELDGGLGSCQALEDHSALAETQEDRASATPRLADSGSVPHDSQVAEGPSVDTRPKKMEKEPAARGTPGTGKERLKAGASPRSVPARKKAQTAPPLQPPPPPPALSEELPWGDLSLNKCLVLASLVALLGSAFQLCRDAVPGEAALQARVPEPWVPPSSAPREPSSPLPKFEAQAPPSAPPAPRAEAEVRPKIPGSREAAENDEEEPGEATGEAVREDRVTLADRGPKERPRREGKPRKEKPRKEERPKKERPRKEERPRAAREPREALPQRWESREGGHRPWARDSRDAEPRKKQAWVSPRRPDEEQRPGSRQKLRAGKGRD

相同性解析による交差性が予測される生物種
Full Name junctional sarcoplasmic reticulum protein 1
Calculated molecular weight 36 kDa, 45 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC021201
Gene Symbol JP45/JSRP1
Gene ID (NCBI) 126306
RRIDAB_2129459
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MG2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JSRP1 (Junctional sarcoplasmic reticulum protein 1), also known as JP-45, is an integral protein of the junctional face membrane of the skeletal muscle sarcoplasmic reticulum (SR), and interacts with calsequestrin and the skeletal-muscle dihydropyridine receptor Cav1. JP-45 is an important protein for functional expression of voltage-dependent calcium channels. Involvement of JP-45 in the molecular mechanism underlying skeletal muscle excitation-contraction coupling has been reported. Although the predicated molecular weight of JP-45 is 36 kDa, it appears a 45-kDa band in western blotting in most cases.

Protocols

Product Specific Protocols
IF protocol for JP45/JSRP1 antibody 10854-1-APDownload protocol
IHC protocol for JP45/JSRP1 antibody 10854-1-APDownload protocol
WB protocol for JP45/JSRP1 antibody 10854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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