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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using JPH1 Polyclonal antibody (25979-1-AP)

    WB analysis of HEK-293 using 25979-1-AP (same clone as 25979-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25979-1-AP (JPH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25979-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using JPH1 Polyclonal antibody (25979-1-AP)

    WB analysis using 25979-1-AP (same clone as 25979-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25979-1-AP (JPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using JPH1 Polyclonal antibody (25979-1-AP)

    IHC staining of human skeletal muscle using 25979-1-AP (same clone as 25979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using JPH1 Polyclonal antibody (25979-1-AP)

    IHC staining of human skeletal muscle using 25979-1-AP (same clone as 25979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using JPH1 Polyclonal antibody (25979-1-AP)

    IHC staining of human skin using 25979-1-AP (same clone as 25979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using JPH1 Polyclonal antibody (25979-1-AP)

    IHC staining of human skin using 25979-1-AP (same clone as 25979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25979-1-PBS in a different storage buffer formulation.

JPH1 Polyclonal antibody, PBS Only

JPH1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25979-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25979-1-PBS targets JPH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23270

Product name: Recombinant human JPH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 464-639 aa of BC140875

Sequence: RSPEASPKHSHSPASSPKPLKKQNPSSGARLNQDKRSVADEQVTAIVNKPLMSKAPTKEAGAVVPQSKYSGRHHIPNPSNGELHSQYHGYYVKLNAPQHPPVDVEDGDGSSQSSSALVHKPSANKWSPSKSVTKPVAKESKAEPKAKKSELAIPKNPASNDSCPALEKEANSGPNS

相同性解析による交差性が予測される生物種
Full Name junctophilin 1
Calculated molecular weight 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC140875
Gene Symbol JPH1
Gene ID (NCBI) 56704
RRIDAB_2880317
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HDC5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Junctophilin-1 is a protein that in humans is encoded by the JPH1 gene. This gene is a member of the junctophilin gene family. Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane.

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