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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

JNK Recombinant antibody, PBS Only

JNK Recombinant Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6J16

Cat no : 81629-1-PBS

Synonyms

c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

Validation Data Gallery

Applications:
  • WB analysis using 81629-1-RR (same clone as 81629-1-PBS)

    WB analysis using 81629-1-RR (same clone as 81629-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81629-1-RR (JNK antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81629-1-RR (same clone as 81629-1-PBS)

    IHC staining of human colon cancer using 81629-1-RR (same clone as 81629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81629-1-RR (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81629-1-RR (same clone as 81629-1-PBS)

    IHC staining of human colon cancer using 81629-1-RR (same clone as 81629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81629-1-RR (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81629-1-RR (same clone as 81629-1-PBS)

    IF Staining of HeLa using 81629-1-RR (same clone as 81629-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JNK antibody (81629-1-RR, Clone: 6J16 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 81629-1-RR (same clone as 81629-1-PBS)

    IF Staining of Jurkat using 81629-1-RR (same clone as 81629-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (81629-1-RR, Clone: 6J16 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 81629-1-RR (same clone as 81629-1-PBS)

    FC experiment of HeLa using 81629-1-RR (same clone as 81629-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human JNK (81629-1-RR, Clone:6J16) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

81629-1-PBS targets JNK in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen JNK fusion protein Ag1639 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 8
Calculated molecular weight 48 kDa
Observed molecular weight44-48 kDa, 50-55 kDa
GenBank accession numberBC130572
Gene symbol MAPK8
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has some isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ). This antibody can recognize JNK1, JNK2 and JNK3.