Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells, U2OS cells |
| Positive IHC detected in | human thyroid cancer tissue, human urothelial carcinoma tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21168-1-AP targets JMJD7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15371 Product name: Recombinant human JMJD7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC139899 Sequence: MAEAALEAVRSELREFPAAARELCVPLAVPYLDKPPTPLHFYRDWVCPNRPCIIRNALQHWPALQKWSLPYFRATVGSTEVSVAVTPDGYADAVRGDRFMMPAERRLPLSFVLDVLEGRAQHPGVLYVQKQCSNLPSELPQLLPDLESHVPWASEALGKMPDAVNFWLGEAAAVTSLHKDHYENLYCVVSGEKHFLFHPPSDRPFIPYELYTPATYQLTEEGTFKVVDEEAMEKVPWIPLDPLAPDLARYPSYSQAQALRCTVRAGEMLYLPALWFHHVQQSQGCIAVNFWYDMEYDLKYSYFQLLDSLTKASGLD 相同性解析による交差性が予測される生物種 |
| Full Name | jumonji domain containing 7 |
| Calculated molecular weight | 316 aa, 36 kDa |
| Observed molecular weight | 35-40 kDa，60-70 kDa |
| GenBank accession number | BC139899 |
| Gene Symbol | JMJD7 |
| Gene ID (NCBI) | 100137047 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0C870 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
JMJD 7 (Jumongji domain containing 7) is a protein containing JmjC domain, belonging to 2- ketoglutarate (2-OG)-dependent dioxygenase family. As an important epigenetic regulatory factor, JMJD7 plays a key role in many physiological and pathological processes such as gene transcription, cell metabolism and bone development (PMID: 41922800). Early biochemical and biophysical studies show that JMJD 7 has terminal endoprotease and aminopeptidase activities, and can target and cut the tail of histone with methylated arginine (such as H2, H3 or H4), thus participating in regulating the stability and transcription of nucleosomes. Abnormal expression of JMJD7 in various solid tumors. For example, it often fuses with phosphatase PLA2G4B, and participates in the development and progress of head and neck squamous cell carcinoma and other malignant tumors. It can also form homodimer (PMID: 29915238).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for JMJD7 antibody 21168-1-AP | Download protocol |
| IHC protocol for JMJD7 antibody 21168-1-AP | Download protocol |
| WB protocol for JMJD7 antibody 21168-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |