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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using JMJD6 Polyclonal antibody (16476-1-AP)

    WB analysis of mouse liver using 16476-1-AP (same clone as 16476-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16476-1-AP (JMJD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using JMJD6 Polyclonal antibody (16476-1-AP)

    IP experiment of K-562 using 16476-1-AP (same clone as 16476-1-PBS)

    IP result of anti-JMJD6 (IP:16476-1-AP, 4ug; Detection:16476-1-AP 1:500) with K-562 cells lysate 1200ug. This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using JMJD6 Polyclonal antibody (16476-1-AP)

    IHC staining of mouse heart using 16476-1-AP (same clone as 16476-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using JMJD6 Polyclonal antibody (16476-1-AP)

    IHC staining of mouse heart using 16476-1-AP (same clone as 16476-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using JMJD6 Polyclonal antibody (16476-1-AP)

    IHC staining of human heart using 16476-1-AP (same clone as 16476-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using JMJD6 Polyclonal antibody (16476-1-AP)

    IHC staining of human heart using 16476-1-AP (same clone as 16476-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using JMJD6 Polyclonal antibody (16476-1-AP)

    FC experiment of A549 using 16476-1-AP (same clone as 16476-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug JMJD6 Polyclonal antibody (16476-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

JMJD6 Polyclonal antibody, PBS Only

JMJD6 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 16476-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

Lysyl-hydroxylase JMJD6, KIAA0585, Jumonji domain-containing protein 6, JmjC domain-containing protein 6, EC:1.14.11.-

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16476-1-PBS targets JMJD6 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9590

Product name: Recombinant human JMJD6 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-403 aa of BC066654

Sequence: MNHKSKKRIREAKRSARPELKDSLDWTRHNYYESFSLSPAAVADNVERADALQLSVEEFVERYERPYKPVVLLNAQEGWSAQEKWTLERLKRKYRNQKFKCGEDNDGYSVKMKMKYYIEYMESTRDDSPLYIFDSSYGEHPKRRKLLEDYKVPKFFTDDLFQYAGEKRRPPYRWFVMGPPRSGTGIHIDPLGTSAWNALVQGHKRWCLFPTSTPRELIKVTRDEGGNQQDEAITWFNVIYPRTQLPTWPPEFKPLEILQKPGETVFVPGGWWHVVLNLDTTIAITQNFASSTNFPVVWHKTVRGRPKLSRKWYRILKQEHPELAVLADSVDLQESTGIASDSSSDSSSSSSSSSSDSDSECESGSEGDGTVHRRKKRRTCSMVGNGDTTSQDDCVSKERSSSR

相同性解析による交差性が予測される生物種
Full Name jumonji domain containing 6
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC066654
Gene Symbol JMJD6
Gene ID (NCBI) 23210
RRIDAB_2265134
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NYC1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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