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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JMJD1C Polyclonal antibody (26637-1-AP)

    WB analysis using 26637-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26637-1-AP (JMJD1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JMJD1C Polyclonal antibody (26637-1-AP)

    IF Staining of HeLa using 26637-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JMJD1C antibody (26637-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

JMJD1C Polyclonal antibody

JMJD1C Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26637-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

TRIP8, TRIP 8, JHDM2C

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-251 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26637-1-AP targets JMJD1C in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24348

Product name: Recombinant human JMJD1C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-138 aa of BC143722

Sequence: TGIITHHDLFTRTMIVMNDQVLEPQNVDPSMVQMTFLDDVVHSLLKGENIGITSRRRSRANQNVNAVHSHYTRAQANSPRPAMNSQAAVPKQNTHQQQQQRSIRPNKRKGSDSS

相同性解析による交差性が予測される生物種
Full Name jumonji domain containing 1C
Calculated molecular weight 2358 aa, 263 kDa
Observed molecular weight285 kDa
GenBank accession numberBC143722
Gene Symbol JMJD1C
Gene ID (NCBI) 221037
RRIDAB_3669557
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15652
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JMJD1C, a member of the lysine demethylase 3 (KDM3) family, is universally required for the survival of several types of acute myeloid leukemia (AML) cells with different genetic mutations.

Protocols

Product Specific Protocols
IF protocol for JMJD1C antibody 26637-1-APDownload protocol
WB protocol for JMJD1C antibody 26637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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