Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34226-1-AP targets JAM2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg6328 Product name: Recombinant mouse CD322/JAM-B/VE-JAM protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 29-236 aa of NM_023844.5 Sequence: FSASKDHRQEVTVIEFQEAILACKTPKKTTSSRLEWKKVGQGVSLVYYQQALQGDFKDRAEMIDFNIRIKNVTRSDAGEYRCEVSAPTEQGQNLQEDKVMLEVLVAPAVPACEVPTSVMTGSVVELRCQDKEGNPAPEYIWFKDGTSLLGNPKGGTHNNSSYTMNTKSGILQFNMISKMDSGEYYCEARNSVGHRRCPGKRMQVDVLN 相同性解析による交差性が予測される生物種 |
| Full Name | junction adhesion molecule 2 |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | NM_023844.5 |
| Gene Symbol | Jam2 |
| Gene ID (NCBI) | 67374 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9JI59 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Junctional adhesion molecules (JAMs) are integral membrane proteins belonging to the immunoglobulin (Ig) superfamily. JAMs are expressed by leukocytes, platelets, endothelial, and epithelial cells and localized at the tight junction of polarized cells and on the cell surface of leukocytes. JAM-2, also known as VE-JAM or JAM-B, is specifically expressed in lymphatic endothelial cells and endothelial venules. JAM2 regulates cell-cell adhesion and signaling by interacting with other tight junction proteins such as PAR-3 and ZO-1 (PMID: 14657270; 24595739; 11739175; 12953056).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for JAM2 antibody 34226-1-AP | Download protocol |
| IHC protocol for JAM2 antibody 34226-1-AP | Download protocol |
| WB protocol for JAM2 antibody 34226-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |