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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using JAM-A/CD321 Recombinant antibody (84720-2-RR)

    WB analysis of HUVEC using 84720-2-RR (same clone as 84720-2-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 84720-2-RR (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84720-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using JAM-A/CD321 Recombinant antibody (84720-2-RR)

    WB analysis of human peripheral blood platelets using 84720-2-RR (same clone as 84720-2-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 84720-2-RR (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84720-2-PBS in a different storage buffer formulation.

JAM-A/CD321 Recombinant monoclonal antibody, PBS Only

JAM-A/CD321 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84720-2-PBS
Clone No.242290C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

F11R, JAM-A/Junctional Adhesion Molecule A, 242290C2, CD321, F11 receptor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84720-2-PBS targets JAM-A/CD321 in WB, Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1372

Product name: Recombinant Human JAM-A/Junctional Adhesion Molecule A protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 28-238 aa of NM_016946.6

Sequence: SVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGV

相同性解析による交差性が予測される生物種
Full Name F11 receptor
Calculated molecular weight33kDa
Observed molecular weight38 kDa
GenBank accession numberNM_016946.6
Gene Symbol F11R
Gene ID (NCBI) 50848
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y624-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and a ligand for the integrin LFA1.