Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, mouse lung tissue, rat lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32747-1-AP targets JAM-A/CD321 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | JAM-A/CD321 fusion protein Eg3117 相同性解析による交差性が予測される生物種 |
| Full Name | F11 receptor |
| Calculated molecular weight | 32 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | NM_172647.2 |
| Gene Symbol | F11r |
| Gene ID (NCBI) | 16456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O88792 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
F11R, also known as Junctional Adhesion Molecule-A (JAM-A), is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is primarily located in the tight junctions of epithelial and endothelial cells and is also expressed on circulating platelets and leukocytes (PMID: 34533648). Targeting F11R/JAM-A with monoclonal antibodies or peptide antagonists has shown promise in inhibiting tumor growth and metastasis in mouse models (PMID: 37563645).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for JAM-A/CD321 antibody 32747-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |