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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using JAK2 Polyclonal antibody (17670-1-AP)

    WB analysis of NIH/3T3 using 17670-1-AP (same clone as 17670-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17670-1-AP (JAK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using JAK2 Polyclonal antibody (17670-1-AP)

    IP experiment of NIH/3T3 using 17670-1-AP (same clone as 17670-1-PBS)

    IP result of anti-JAK2 (IP:17670-1-AP, 4ug; Detection:17670-1-AP 1:300) with NIH/3T3 cells lysate 3440 ug. This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using JAK2 Polyclonal antibody (17670-1-AP)

    IHC staining of human kidney using 17670-1-AP (same clone as 17670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using JAK2 Polyclonal antibody (17670-1-AP)

    IHC staining of human kidney using 17670-1-AP (same clone as 17670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using JAK2 Polyclonal antibody (17670-1-AP)

    IHC staining of human breast cancer using 17670-1-AP (same clone as 17670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using JAK2 Polyclonal antibody (17670-1-AP)

    IHC staining of human breast cancer using 17670-1-AP (same clone as 17670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

JAK2 Polyclonal antibody, PBS Only

JAK2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17670-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

Tyrosine protein kinase JAK2, JTK10, Janus kinase 2, JAK-2, JAK 2

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17670-1-PBS targets JAK2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11264

Product name: Recombinant human JAK2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 764-1129 aa of BC039695

Sequence: EDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG

相同性解析による交差性が予測される生物種
Full Name Janus kinase 2 (a protein tyrosine kinase)
Calculated molecular weight 1132 aa, 130 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC039695
Gene Symbol JAK2
Gene ID (NCBI) 3717
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60674
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer.

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