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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JAK1 Polyclonal antibody (27284-1-AP)

    WB analysis using 27284-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27284-1-AP (JAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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JAK1 Polyclonal antibody

JAK1 Polyclonal Antibody for WB, ELISA
Cat No. 27284-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:2.7.10.2, JAK 1, JAK-1, JAK1A, JAK1B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDaudi cells, J774A.1 cells, RAW 264.7 cells, mouse lung tissue, rat lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27284-1-AP targets JAK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JAK1 fusion protein Ag25579 相同性解析による交差性が予測される生物種
Full Name Janus kinase 1 (a protein tyrosine kinase)
Calculated molecular weight 1154 aa, 133 kDa
Observed molecular weight125-133 kDa
GenBank accession numberBC132729
Gene Symbol JAK1
Gene ID (NCBI) 3716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23458
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.

Protocols

Product Specific Protocols
WB protocol for JAK1 antibody 27284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols