Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells, C6 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34193-1-AP targets JAGN1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40789 Product name: Recombinant human JAGN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-39 aa of BC032101 Sequence: MASRAGPRAAGTDGSDFQHRERVAMHYQMSVTLKYEIKK 相同性解析による交差性が予測される生物種 |
| Full Name | jagunal homolog 1 (Drosophila) |
| Calculated molecular weight | 183 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC032101 |
| Gene Symbol | JAGN1 |
| Gene ID (NCBI) | 84522 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N5M9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Jagunal homolog 1 (JAGN1) is an evolutionarily conserved endoplasmic reticulum (ER) membrane protein essential for neutrophil differentiation and function. First identified in Drosophila for its role in ER organization, human JAGN1 mutations cause severe congenital neutropenia type 5, characterized by recurrent infections due to impaired granulocyte development. JAGN1 regulates glycoprotein folding, ER-Golgi trafficking, and N-glycosylation of proteins critical for neutrophil viability. Loss of JAGN1 leads to ER stress, apoptosis of myeloid progenitors, and defective neutrophil migration. Its indispensable role in innate immunity highlights JAGN1 as a potential therapeutic target for neutropenia and immunodeficiency disorders (PMID: 25129144; 37120535; 33207009; 38739706).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for JAGN1 antibody 34193-1-AP | Download protocol |
| WB protocol for JAGN1 antibody 34193-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |