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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Islet 1 Recombinant antibody (83784-4-RR)

    WB analysis using 83784-4-RR (same clone as 83784-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83784-4-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Islet 1 Recombinant antibody (83784-4-RR)

    IHC staining of mouse brain using 83784-4-RR (same clone as 83784-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83784-4-RR (Islet 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Islet 1 Recombinant antibody (83784-4-RR)

    IHC staining of mouse brain using 83784-4-RR (same clone as 83784-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83784-4-RR (Islet 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Islet 1 Recombinant antibody (83784-4-RR)

    IF Staining of SH-SY5Y using 83784-4-RR (same clone as 83784-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Islet 1 antibody (83784-4-RR, Clone: 240833D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83784-4-RR

    Affinity and Kinetic Characterization of 83784-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83784-4-RR against Human Islet 1 were performed. The affinity constant is below 1 pM.

Islet 1 Recombinant monoclonal antibody, PBS Only

Islet 1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83784-4-PBS
Clone No.240833D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

ISL1, 240833D7, Insulin gene enhancer protein ISL-1, Isl 1, ISL LIM homeobox 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83784-4-PBS targets Islet 1 in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag33541

Product name: Recombinant human Islet 1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-349 aa of BC031213

Sequence: MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKCAECNQYLDESCTCFVRDGKTYCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPISARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPNSMVASPIEA*

相同性解析による交差性が予測される生物種
Full Name ISL LIM homeobox 1
Calculated molecular weight 39 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberBC031213
Gene Symbol Islet 1
Gene ID (NCBI) 3670
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP61371
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.