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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using Intersectin 1 Polyclonal antibody (21862-1-AP)

    WB analysis of fetal human brain using 21862-1-AP (same clone as 21862-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21862-1-AP (Intersectin 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21862-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Intersectin 1 Polyclonal antibody (21862-1-AP)

    IHC staining of human stomach using 21862-1-AP (same clone as 21862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21862-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Intersectin 1 Polyclonal antibody (21862-1-AP)

    IHC staining of human stomach using 21862-1-AP (same clone as 21862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21862-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Intersectin 1 Polyclonal antibody (21862-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 21862-1-AP (same clone as 21862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21862-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Intersectin 1 Polyclonal antibody (21862-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 21862-1-AP (same clone as 21862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21862-1-PBS in a different storage buffer formulation.

Intersectin 1 Polyclonal antibody, PBS Only

Intersectin 1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21862-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

ITSN1, SH3 domain-containing protein 1A, ITSN 1, ITSN, Intersectin-1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21862-1-PBS targets Intersectin 1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16517

Product name: Recombinant human ITSN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 610-926 aa of BC116186

Sequence: LREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRA

相同性解析による交差性が予測される生物種
Full Name intersectin 1 (SH3 domain protein)
Calculated molecular weight 1721 aa, 195 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC116186
Gene Symbol Intersectin 1
Gene ID (NCBI) 6453
RRIDAB_2878931
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15811
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Intersectin 1 (ITSN1) is a multidomain adaptor protein that plays a crucial role in various cellular processes, particularly in the nervous system. It is involved in clathrin-mediated endocytosis, signal transduction, and the regulation of the actin cytoskeleton. The protein is highly abundant in neurons and has been implicated in neurodevelopmental disorders, such as autism spectrum disorders, intellectual disability, and epilepsy. Recent studies have identified de novo variants in the ITSN1 gene in patients with these disorders, suggesting a link between ITSN1 and brain development.

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