Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IF/ICC detected in | HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 24 publications below |
| IHC | See 10 publications below |
| IF | See 8 publications below |
Product Information
27506-1-AP targets IFN-beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26326 Product name: Recombinant human IFNB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-187 aa of BC069314 Sequence: MSYNLLGFLQRSSNFQCQKLLWQLNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNETIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYSHCAWTIVRVEILRNFYFINRLTGYLRN 相同性解析による交差性が予測される生物種 |
| Full Name | IFN beta |
| Calculated molecular weight | 187 aa, 22 kDa |
| GenBank accession number | BC069314 |
| Gene Symbol | IFN beta1 |
| Gene ID (NCBI) | 3456 |
| RRID | AB_2880893 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01574 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interferon-beta (IFN-β) is a type I interferon, which is a group of signaling proteins made and released by host cells in response to the presence of several viruses, including double-stranded RNA produced by many viruses. IFN-β is a single protein species and is molecularly related to IFN-α subtypes, although it is antigenically distinct from them. It is mainly produced by fibroblasts and plays a crucial role in the innate immune response against viruses. IFN-β can inhibit viral replication by interfering with the virus's RNA or DNA, thus suppressing viral growth. It also enhances the cytotoxic activity of natural killer (NK) cells and promotes the expression of major histocompatibility complex (MHC) class I molecules.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IFN-beta antibody 27506-1-AP | Download protocol |
| WB protocol for IFN-beta antibody 27506-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res GFER Represents a Target for Dual Disruption of Redox Homeostasis and Reactivation of the Immune Response in Pancreatic Adenocarcinoma. | ||
Nat Commun Airway immune profiles and therapeutic implications of IGF1 in eosinophilic granulomatosis with polyangiitis. | ||
Int J Oral Sci Targeting IFN-I driven tertiary lymphoid structures halts B cell-mediated bone loss in periodontitis. | ||
Mol Cell hnRNPM protects against the dsRNA-mediated interferon response by repressing LINE-associated cryptic splicing | ||
Acta Pharm Sin B A new approach to discovering a broad-spectrum anti-respiratory virus drug: Tubeimoside II modulates host factor PACT to potentiate the RIG-I antiviral pathway.
| ||
Adv Sci (Weinh) Inhibition of CDH11 Activates cGAS-STING by Stimulating Branched Chain Amino Acid Catabolism and Mitigates Lung Metastasis of Adenoid Cystic Carcinoma |