Validation Data Gallery
Tested Applications
| Positive WB detected in | Neuro-2a cells, C6 cells, mouse lung tissue |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87465-1-RR targets Integrin beta 5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg10456 Product name: Recombinant mouse ITB5 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-Twin-Strep-Tag Sequence: \ 相同性解析による交差性が予測される生物種 |
| Full Name | integrin beta 5 |
| Observed molecular weight | 100 kDa |
| Gene Symbol | Itgb5 |
| Gene ID (NCBI) | 16419 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrin beta-5 (ITGB5) is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Integrin beta 5 antibody 87465-1-RR | Download protocol |
| IHC protocol for Integrin beta 5 antibody 87465-1-RR | Download protocol |
| WB protocol for Integrin beta 5 antibody 87465-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |