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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin beta 4 Recombinant monoclonal antibody (87416-2-RR)

    WB analysis using 87416-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87416-2-RR (ITB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity of 87416-2-RR

    Affinity of 87416-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87416-2-RR against Human Integrin beta 4 were performed. The affinity constant is 6.72 nM.

Integrin beta 4 Recombinant monoclonal antibody

Integrin beta 4 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87416-2-RR
Clone No.252740B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ITGB4, CD104, GP150, Integrin beta-4, integrin, beta 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, mouse skin tissue, rat skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87416-2-RR targets Integrin beta 4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg10479

Product name: Recombinant human ITB4 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 10011436 of

Sequence: \

相同性解析による交差性が予測される生物種
Full Name integrin, beta 4
Observed molecular weight 200 kDa
Gene Symbol Integrin beta 4
Gene ID (NCBI) 3691
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Protocols

Product Specific Protocols
WB protocol for Integrin beta 4 antibody 87416-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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