Validation Data Gallery

Filter: AllWBIHC

WB analysis using 26918-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26918-1-AP (Integrin Beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 26918-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human skin using 26918-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skin using 26918-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human lung cancer using 26918-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 26918-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Integrin Beta 1/CD29 Polyclonal antibody

Integrin Beta 1/CD29 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26918-1-AP

Publications(40)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

ITGB1, CD29, FNRB, Glycoprotein IIa, GPIIA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (40)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse lung tissue, HT-1080 cells, rat lung tissue
Positive IHC detected in	human skin tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 30 publications below
IHC	See 4 publications below

Product Information

26918-1-AP targets Integrin Beta 1/CD29 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25426 Product name: Recombinant human Integrin beta-1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 752-798 aa of BC020057 Sequence: KLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK 相同性解析による交差性が予測される生物種
Full Name	integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated molecular weight	88 kDa
Observed molecular weight	130 kDa
GenBank accession number	BC020057
Gene Symbol	Integrin beta 1
Gene ID (NCBI)	3688
ENSEMBL Gene ID	ENSG00000150093
RRID	AB_2880685
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05556
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for Integrin Beta 1/CD29 antibody 26918-1-AP	Download protocol
WB protocol for Integrin Beta 1/CD29 antibody 26918-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Bioact Mater Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing Authors - Bingcheng Yi View Article
human	WB	Nat Commun Regulation of local GTP availability controls RAC1 activity and cell invasion Authors - Anna Bianchi-Smiraglia View Article
rat	WB	Theranostics A functionalized self-assembling peptide containing E7 and YIGSR sequences enhances neuronal differentiation of spermatogonial stem cells on aligned PCL fibers for spinal cord injury repair Authors - Zhiyuan Wang View Article
human	WB	Mater Today Bio Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy. Authors - Lili Liu View Article
rat	WB	ACS Appl Mater Interfaces Engineered Biomimetic Nanoplatform Protects the Myocardium Against Ischemia/Reperfusion Injury by Inhibiting Pyroptosis. Authors - Yazhong Wei View Article
human	WB	Cancer Lett YTHDF1 promotes the osteolytic bone metastasis of breast cancer via inducing EZH2 and CDH11 translation Authors - Shuoer Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26918-1-AP

WB analysis of HT-1080 using 26918-1-AP

IHC staining of human skin using 26918-1-AP

IHC staining of human skin using 26918-1-AP

IHC staining of human lung cancer using 26918-1-AP

IHC staining of human lung cancer using 26918-1-AP

Integrin Beta 1/CD29 Polyclonal antibody

Integrin Beta 1/CD29 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing

Regulation of local GTP availability controls RAC1 activity and cell invasion

A functionalized self-assembling peptide containing E7 and YIGSR sequences enhances neuronal differentiation of spermatogonial stem cells on aligned PCL fibers for spinal cord injury repair

Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy.

Engineered Biomimetic Nanoplatform Protects the Myocardium Against Ischemia/Reperfusion Injury by Inhibiting Pyroptosis.

YTHDF1 promotes the osteolytic bone metastasis of breast cancer via inducing EZH2 and CDH11 translation