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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human PBMCs using Integrin alpha-E Monoclonal antibody (68893-3-Ig)

    IF Staining of human PBMCs using 68893-3-Ig (same clone as 68893-3-PBS)

    Immunofluorescent analysis of un-fixed 5 µg/mL PHA ( 3 days) treated human PBMCs using Integrin alpha-E antibody (68893-3-Ig, Clone: 4B6G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). This data was developed using the same antibody clone with 68893-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Integrin alpha-E Monoclonal antibody (68893-3-Ig)

    FC experiment of human PBMCs using 68893-3-Ig (same clone as 68893-3-PBS)

    1x10^6 untreated human PBMCs (left) and 5 µg/mL PHA (3 days) treated human PBMCs (right) were surface stained with 0.2 μg Integrin Alpha-E Monoclonal Antibody (68893-3-Ig, Clone: 4B6G6) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Cells were not fixed. This data was developed using the same antibody clone with 68893-3-PBS in a different storage buffer formulation.

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Integrin alpha-E Monoclonal antibody, PBS Only

Integrin alpha-E Monoclonal Antibody for IF/ICC, FC, Indirect ELISA
Cat No. 68893-3-PBS
Clone No.4B6G6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC, Indirect ELISA

Synonyms

CD103, 4B6G6, Integrin alpha E, HUMINAE, HML-1 antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68893-3-PBS targets Integrin alpha-E in IF/ICC, FC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Integrin alpha-E fusion protein Ag27410 相同性解析による交差性が予測される生物種
Full Name integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
Calculated molecular weight 1179 aa, 130 kDa
GenBank accession numberBC113436
Gene Symbol ITGAE
Gene ID (NCBI) 3682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G Magarose purification
UNIPROT IDP38570
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Integrin alpha-E (CD103) is a member of the integrin family, specifically the αEβ7 heterodimer. It plays a crucial role in the immune system, particularly in the interaction between leukocytes and epithelial cells. Integrin alpha-E (CD103) is a critical component of the immune system, particularly in the context of mucosal immunity. Its ability to bind to E-cadherin and regulate the production of IFN-I in cDCs during viral infections highlights its multifaceted role in immune regulation and disease prevention. (PMID: 33584680)