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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin alpha-8 Recombinant monoclonal antibody (87408-1-RR)

    WB analysis using 87408-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87408-1-RR (Integrin alpha-8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Integrin alpha-8 Recombinant monoclonal antibody (87408-1-RR)

    WB analysis using 87408-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87408-1-RR (Integrin alpha-8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Integrin alpha-8 Recombinant monoclonal antibody (87408-1-RR)

    WB analysis using 87408-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87408-1-RR (Integrin alpha-8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using Integrin alpha-8 Recombinant monoclonal antibody (87408-1-RR)

    IF Staining of mouse lung using 87408-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Integrin alpha-8 antibody (87408-1-RR, Clone: 252745D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using Integrin alpha-8 Recombinant monoclonal antibody (87408-1-RR)

    IF Staining of mouse lung using 87408-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Integrin alpha-8 antibody (87408-1-RR, Clone: 252745D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 87408-1-RR

    Affinity of 87408-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87408-1-RR against Mouse Integrin alpha 8 were performed. The affinity constant is 2.24 nM.

Integrin alpha-8 Recombinant monoclonal antibody

Integrin alpha-8 Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 87408-1-RR
Clone No.252745D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

Integrin alpha-8 heavy chain, Integrin alpha-8 light chain, Itga8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue, human kidney tissue, mouse lung tissue, mouse kidney tissue, rat lung tissue, rat spleen tissue, rat kidney tissue
Positive IF-P detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87408-1-RR targets Integrin alpha-8 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg10469

Product name: Recombinant mouse ITA8 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 10011446 of

Sequence: \

相同性解析による交差性が予測される生物種
Full Name integrin alpha 8
Calculated molecular weight117 kDa
Observed molecular weight140 kDa
Gene Symbol Itga8
Gene ID (NCBI) 241226
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDA2ARA8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).

Protocols

Product Specific Protocols
IF protocol for Integrin alpha-8 antibody 87408-1-RRDownload protocol
WB protocol for Integrin alpha-8 antibody 87408-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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