• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Integrin alpha-5 Recombinant antibody (84468-4-RR)

    WB analysis using 84468-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84468-4-RR (Integrin alpha-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HAP1 cells using Integrin alpha-5/CD49e Recombinant antibody (84468-4-RR)

    WB analysis of HAP1 using 84468-4-RR

    WB result of Integrin alpha-5/CD49e antibody (84468-4-RR; 1:4000; room temperature for 1.5 hours) with wild-type and sg-Integrin alpha-5/CD49e transfected HAP1 cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Integrin alpha-5 Recombinant antibody (84468-4-RR)

    IF Staining of HeLa using 84468-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Integrin alpha-5 antibody (84468-4-RR, Clone: 241685A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84468-4-RR

    Affinity and Kinetic Characterization of 84468-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84468-4-RR against Human Integrin alpha-5 were performed. The affinity constant is 1.24 nM.

  • Featured Product
  • KD/KO Validated

Integrin alpha-5/CD49e Recombinant monoclonal antibody

Integrin alpha-5/CD49e Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84468-4-RR
Clone No.241685A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Integrin Alpha-5, ITGA5, 241685A3, CD49 antigen-like family member E, CD49e

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HAP1 cells, HeLa cells, NIH/3T3 cells, human placenta tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84468-4-RR targets Integrin alpha-5/CD49e in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant Protein

相同性解析による交差性が予測される生物種
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Calculated molecular weight 115 kDa
Observed molecular weight140 kDa
GenBank accession numberBC008786
Gene Symbol Integrin alpha 5
Gene ID (NCBI) 3678
RRIDAB_3671991
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP08648
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

Protocols

Product Specific Protocols
IF protocol for Integrin alpha-5/CD49e antibody 84468-4-RRDownload protocol
WB protocol for Integrin alpha-5/CD49e antibody 84468-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...