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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin alpha 11 Recombinant antibody (84695-1-RR)

    WB analysis using 84695-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84695-1-RR (Integrin alpha 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Integrin alpha 11 Recombinant antibody

Integrin alpha 11 Recombinant Antibody for WB, ELISA
Cat No. 84695-1-RR
Clone No.241351B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ITGA11, Integrin alpha-11, 241351B7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, Neuro-2a cells, HeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84695-1-RR targets Integrin alpha 11 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 11
Calculated molecular weight 133 kDa
Observed molecular weight170 kDa
GenBank accession numberNM_001004439
Gene Symbol ITGA11
Gene ID (NCBI) 22801
RRIDAB_3672111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UKX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha 11 (ITGA11) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen. This receptor is involved in cell migration and collagen reorganization on mesenchymal nonmuscle cells (PMID: 11518510). It is also involved in the regulation of myofibroblast phenotypic differentiation during fibrotic diseases (PMID: 29147013).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha 11 antibody 84695-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols