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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis using 66922-2-Ig (same clone as 66922-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW 1990 cells using Integrin beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis of SW 1990 using 66922-2-Ig (same clone as 66922-2-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Integrin beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis using 66922-2-Ig (same clone as 66922-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using Integrin beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis of BxPC-3 using 66922-2-Ig (same clone as 66922-2-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Integrin beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis of A549 using 66922-2-Ig (same clone as 66922-2-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin beta-4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Integrin beta 4 Monoclonal antibody (66922-2-Ig)

    IHC staining of human skin cancer using 66922-2-Ig (same clone as 66922-2-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Integrin beta 4 Monoclonal antibody (66922-2-Ig)

    IHC staining of human skin cancer using 66922-2-Ig (same clone as 66922-2-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Integrin beta 4 Monoclonal antibody (66922-2-Ig)

    IF Staining of A431 using 66922-2-Ig (same clone as 66922-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 4 antibody (66922-2-Ig, Clone: 1C2A11 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

Integrin beta 4 Monoclonal antibody, PBS Only

Integrin beta 4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66922-2-PBS
Clone No.1C2A11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ITGB4, 1C2A11, CD104, GP150, Integrin beta-4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66922-2-PBS targets Integrin beta 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17395

Product name: Recombinant human Integrin beta-4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 27-354 aa of BC126411

Sequence: ANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRRCNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFELEVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYRTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSN

相同性解析による交差性が予測される生物種
Full Name integrin, beta 4
Calculated molecular weight 1752 aa, 195 kDa
Observed molecular weight 200-210 kDa
GenBank accession numberBC126411
Gene Symbol Integrin beta 4
Gene ID (NCBI) 3691
RRIDAB_2882249
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP16144
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

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