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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Integrin Alpha 6 Monoclonal antibody

Integrin Alpha 6 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B12A1

Cat no : CL488-66906

Synonyms

CD49f, DKFZp686J01244, FLJ18737, Integrin alpha 6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6

Validation Data Gallery

Applications:
  • FC experiment of A431 using CL488-66906

    FC experiment of A431 using CL488-66906

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Integrin Alpha 6 (CL488-66906, Clone:2B12A1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 66906-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66906 targets Integrin Alpha 6 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Integrin Alpha 6 fusion protein Ag25866 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 6
Calculated molecular weight 1073 aa, 119 kDa
Observed molecular weight 115-120 kDa
GenBank accession numberBC136456
Gene symbol ITGA6
Gene ID (NCBI) 3655
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Integrin Alpha 6 antibody CL488-66906Download protocol
Standard Protocols
Click here to view our Standard Protocols