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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IkB Alpha Recombinant antibody

IkB Alpha Recombinant Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

5C24

Cat no : 80019-1-RR

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

Validation Data Gallery

Applications:
  • WB analysis using 80019-1-RR

    WB analysis using 80019-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80019-1-RR (IkB alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 80019-1-RR

    IHC staining of human breast cancer using 80019-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFKBIA antibody included
  • IHC staining of human breast cancer using 80019-1-RR

    IHC staining of human breast cancer using 80019-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFKBIA antibody included
  • IHC staining of human prostate cancer using 80019-1-RR

    IHC staining of human prostate cancer using 80019-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFKBIA antibody included
  • IHC staining of human prostate cancer using 80019-1-RR

    IHC staining of human prostate cancer using 80019-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFKBIA antibody included

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, Raji cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

80019-1-RR targets IkB Alpha in WB, IHC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IkB Alpha fusion protein Ag0345 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated molecular weight 36 kDa
Observed molecular weight36 kDa and 35 kDa
GenBank accession numberBC002601
Gene symbol NFKBIA
Gene ID (NCBI) 4792
RRIDAB_2882942
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. IkB alpha can be observed as a band at 36 kDa in human cell lysates, and 35 kDa in mouse and rat cell ysates.

Protocols

Product Specific Protocols
WB protocol for IkB Alpha antibody 80019-1-RRDownload protocol
IHC protocol for IkB Alpha antibody 80019-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols