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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IkB Beta Polyclonal antibody (12660-1-AP)

    WB analysis using 12660-1-AP (same clone as 12660-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using IkB Beta Polyclonal antibody (12660-1-AP)

    WB analysis of MCF-7 using 12660-1-AP (same clone as 12660-1-PBS)

    WB result of IkB Beta antibody (12660-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-IkB Beta transfected MCF-7 cells. This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using IkB Beta Polyclonal antibody (12660-1-AP)

    WB analysis of MCF-7 using 12660-1-AP (same clone as 12660-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human colon using 12660-1-AP (same clone as 12660-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human colon using 12660-1-AP (same clone as 12660-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human kidney using 12660-1-AP (same clone as 12660-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human colon cancer using 12660-1-AP (same clone as 12660-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human endometrial cancer using 12660-1-AP (same clone as 12660-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human endometrial cancer using 12660-1-AP (same clone as 12660-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human kidney using 12660-1-AP (same clone as 12660-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IkB Beta Polyclonal antibody (12660-1-AP)

    IF Staining of HeLa using 12660-1-AP (same clone as 12660-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IkB Beta antibody (12660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12660-1-PBS in a different storage buffer formulation.

IkB Beta Polyclonal antibody, PBS Only

IkB Beta Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12660-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

IκB beta, IκB β, NFKBIB, I kappa B beta, IkappaBbeta

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12660-1-PBS targets IkB Beta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3340

Product name: Recombinant human NFKBIB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-356 aa of BC015528

Sequence: MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV

相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Calculated molecular weight 356 aa, 38 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC015528
Gene Symbol IkB Beta
Gene ID (NCBI) 4793
RRIDAB_2151611
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15653
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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