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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IkB Alpha Monoclonal antibody (66418-1-Ig)

    WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IkB Alpha Monoclonal antibody (66418-1-Ig)

    WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IkB Alpha Monoclonal antibody (66418-1-Ig)

    WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using IkB Alpha Monoclonal antibody (66418-1-Ig)

    WB analysis of HEK-293 using 66418-1-Ig (same clone as 66418-1-PBS)

    WB result of IkB Alpha antibody (66418-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IkB Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using IkB Alpha Monoclonal antibody (66418-1-Ig)

    WB analysis of C6 using 66418-1-Ig (same clone as 66418-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using IkB Alpha Monoclonal antibody (66418-1-Ig)

    WB analysis of ROS1728 using 66418-1-Ig (same clone as 66418-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IkB Alpha Monoclonal antibody (66418-1-Ig)

    IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IkB Alpha Monoclonal antibody (66418-1-Ig)

    IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51027-2

    Cytometric bead array standard curve of MP51027-2

    Cytometric bead array standard curve of MP51027-2, NFKBIA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66418-1-PBS. Detection antibody: 60712-1-PBS. Standard:Ag0345. Range: 0.391-100 ng/mL.

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  • Featured Product
  • KD/KO Validated

IkB Alpha Monoclonal antibody, PBS Only (Capture)

IkB Alpha Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66418-1-PBS
Clone No.2G6H7
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

IKBA, IκB α, NFKBIA, IkB-alpha, I-kappa-B-alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Published Applications

WBSee 1 publications below

Product Information

66418-1-PBS targets IkB Alpha as part of a matched antibody pair:

MP51027-2: 66418-1-PBS capture and 60712-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IkB Alpha fusion protein Ag22040 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004983
Gene Symbol IkB Alpha
Gene ID (NCBI) 4792
RRIDAB_2881790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP25963
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publications

SpeciesApplicationTitle
mouseWB

Neoplasia

ZC3H15 suppression ameliorates bone cancer pain through inhibiting neuronal oxidative stress and microglial inflammation

Authors - Li-Quan Huang
View Article