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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse IgG2b Isotype Control (MPC-11)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Applications

FC

Conjugate

Unconjugated

CloneNo.

MPC-11

Cat no : 65128-1-Ig

Synonyms

IgG2b, IgG2b Isotype Control, Mouse IgG2b Isotype Control

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using 65128-1-Ig

    FC experiment of human PBMCs using 65128-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Mouse IgG2b Isotype Control (65128-1-Ig, Clone:MPC-11) (blue), or Anti-Human CD4 (red) followed by CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained (black). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65128-1-Ig targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy chain 3 (serum IgG2b)
Gene symbol Igh-3
Gene ID (NCBI) 16016
RRIDAB_2918419
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for IgG2b Isotype Control antibody 65128-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols