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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IZUMO1 Polyclonal antibody (13296-1-AP)

    WB analysis using 13296-1-AP (same clone as 13296-1-PBS)

    Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13296-1-AP (IZUMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13296-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using IZUMO1 Polyclonal antibody (13296-1-AP)

    IF Staining of mouse testis using 13296-1-AP (same clone as 13296-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 13296-1-AP (IZUMO1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13296-1-PBS in a different storage buffer formulation.

IZUMO1 Polyclonal antibody, PBS Only

IZUMO1 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 13296-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

IZUMO, Izumo sperm-egg fusion protein 1, OBF, Sperm-specific protein izumo

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13296-1-PBS targets IZUMO1 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4008

Product name: Recombinant human IZUMO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-294 aa of BC034769

Sequence: LALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASRLL

相同性解析による交差性が予測される生物種
Full Name izumo sperm-egg fusion 1
Calculated molecular weight 350 aa, 39 kDa
Observed molecular weight70 kDa
GenBank accession numberBC034769
Gene Symbol IZUMO1
Gene ID (NCBI) 284359
RRIDAB_2877934
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYV9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The Izumo sperm egg fusion gene (IZUMO1) is a crucial member of the immunoglobulin superfamily and plays a crucial role in spermatogenesis, sperm egg interaction, and fusion. The expression of IZUMO1 mRNA was mainly in the testis and sperm and performed an important part in fertilization. IZUMO1 possess a well-conserved N-glycosylation site in the middle of an Ig loop among species. Research indicates that the glycosylated form of IZUMO1 has a molecular weight of 56 kDa, while its dimeric form has a molecular weight of 70 kDa (PMID: 21057513, PMID: 31146500).

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