Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, mouse brain tissue, rat brain tissue, HeLa cells, Jurkat cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| ChIP | See 1 publications below |
Product Information
16943-1-AP targets IWS1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IWS1 fusion protein Ag10554 相同性解析による交差性が予測される生物種 |
| Full Name | IWS1 homolog (S. cerevisiae) |
| Calculated molecular weight | 819 aa, 92 kDa |
| Observed molecular weight | 116-120 kDa |
| GenBank accession number | BC017012 |
| Gene Symbol | IWS1 |
| Gene ID (NCBI) | 55677 |
| RRID | AB_2128229 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96ST2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IWS1, as a transcription elongation factor, functions to recruit cofactors to the RNA polymerase II (RNAPII) complex to regulate mRNA alternative splicing (PMID: 30846735; 37237004). It plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. It also acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (PMID: 17184735; 17234882; 19141475).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IWS1 antibody 16943-1-AP | Download protocol |
| IHC protocol for IWS1 antibody 16943-1-AP | Download protocol |
| IF protocol for IWS1 antibody 16943-1-AP | Download protocol |
| IP protocol for IWS1 antibody 16943-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Host Microbe The Integrase Cofactor LEDGF/p75 Associates with Iws1 and Spt6 for Postintegration Silencing of HIV-1 Gene Expression in Latently Infected Cells. |