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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of mouse heart using 14741-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of mouse ovary using 14741-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of HeLa using 14741-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of HEK-293 using 14741-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Apoptosised HeLa cells using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of Apoptosised HeLa using 14741-1-AP

    Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of mouse ovary using 14741-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of K-562 using 14741-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IP experiment of mouse heart using 14741-1-AP

    IP result of anti-IVNS1ABP (IP:14741-1-AP, 4ug; Detection:14741-1-AP 1:800) with mouse heart tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of human kidney using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14741-1-AP (IVNS1ABP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of human kidney using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14741-1-AP (IVNS1ABP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of human heart using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of human heart using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of mouse kidney using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of mouse kidney using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 40x lens).

IVNS1ABP Polyclonal antibody

IVNS1ABP Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14741-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Kelch-like protein 39, Influenza virus NS1A-binding protein, HSPC068, FLARA3, Aryl hydrocarbon receptor-associated protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, Apoptosised HeLa cells, HEK-293 cells, HeLa cells, K-562 cells, mouse ovary tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14741-1-AP targets IVNS1ABP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6412

Product name: Recombinant human IVNS1ABP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 301-633 aa of BC067739

Sequence: TYLCLAVLDGIFCVIFLHGRNSPQSSPTSTPKLSKSLSFEMQQDELIEKPMSPMQYARSGLGTAEMNGKLIAAGGYNREECLRTVECYNPHTDHWSFLAPMRTPRARFQMAVLMGQLYVVGGSNGHSDDLSCGEMYDSNIDDWIPVPELRTNRCNAGVCALNGKLYIVGGSDPYGQKGLKNCDVFDPVTKLWTSCAPLNIRRHQSAVCELGGYLYIIGGAESWNCLNTVERYNPENNTWTLIAPMNVARRGAGVAVLNGKLFVCGGFDGSHAISCVEMYDPTRNEWKMMGNMTSPRSNAGIATVGNTIYAVGGFDGNEFLNTVEVYNLESNEW

相同性解析による交差性が予測される生物種
Full Name influenza virus NS1A binding protein
Calculated molecular weight 72 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC067739
Gene Symbol IVNS1ABP
Gene ID (NCBI) 10625
RRIDAB_10638784
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Y0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IVNS1ABP is involved in many cell functions, including pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, F-actin organization and protein ubiquitination. It may play a role in mRNA nuclear export.

Protocols

Product Specific Protocols
WB protocol for IVNS1ABP antibody 14741-1-APDownload protocol
IHC protocol for IVNS1ABP antibody 14741-1-APDownload protocol
IP protocol for IVNS1ABP antibody 14741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Global landscape of HIV-human protein complexes.

Authors - Stefanie Jäger
View Article
human,mouseWB

Inflammation

Nonstructural Protein 1 Mediates HMGB1 Release by Targeting Histone H1.0 After Respiratory Syncytial Virus Infection In Vivo and In Vitro

Authors - Na Zhou
View Article