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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IVD Monoclonal antibody (66032-1-Ig)

    WB analysis using 66032-1-Ig (same clone as 66032-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using IVD Monoclonal antibody (66032-1-Ig)

    WB analysis of MCF-7 using 66032-1-Ig (same clone as 66032-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using IVD Monoclonal antibody (66032-1-Ig)

    WB analysis of human liver using 66032-1-Ig (same clone as 66032-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using IVD Monoclonal antibody (66032-1-Ig)

    WB analysis of HEK-293 using 66032-1-Ig (same clone as 66032-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66032-1-Ig(IVD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66032-1-Ig(IVD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IF Staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IF Staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using IVD Monoclonal antibody (66032-1-Ig)

    IF Staining of MCF-7 using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using IVD antibody 66032-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

IVD Monoclonal antibody, PBS Only

IVD Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66032-1-PBS
Clone No.2D12A5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Isovaleryl-CoA dehydrogenase, mitochondrial, EC:1.3.8.4, EC:1.3.8.1, Butyryl-CoA dehydrogenase, ACAD2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66032-1-PBS targets IVD in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag18042

Product name: Recombinant human IVD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-423 aa of BC017202

Sequence: MATATRLLGCRVASWRLRPPLAGFVSQRAHSLLPVDDAINGLSEEQRQLRQTMAKFLQEHLAPKAQEIDRSNEFKNLREFWKQLGNLGVLGITAPVQYGGSGLGYLEHVLVMEEISRASGAVGLSYGAHSNLCINQLVRNGNEAQKEKYLPKLISGEYIGALAMSEPNAGSDVVSMKLKAEKKGNHYILNGNKFWITNGPDADVLIVYAKTDLAAVPASRGITAFIVEKGMPGFSTSKKLDKLGMRGSNTCELIFEDCKIPAANILGHENKGVYVLMSGLDLERLVLAGGPLGLMQAVLDHTIPYLHVREAFGQKIGHFQLMQGKMADMYTRLMACRQYVYNVAKACDEGHCTAKDCAGVILYSAECATQVALDGIQCFGGNGYINDFPMGRFLRDAKLYEIGAGTSEVRRLVIGRAFNADFH

相同性解析による交差性が予測される生物種
Full Name isovaleryl Coenzyme A dehydrogenase
Calculated molecular weight 46 kDa
Observed molecular weight 41-46 kDa
GenBank accession numberBC017202
Gene Symbol IVD
Gene ID (NCBI) 3712
RRIDAB_11042768
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP26440
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.

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