Validation Data Gallery

Filter: AllWBIP

WB analysis of A549 using 26055-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 26055-1-AP (ITPRIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 26055-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 26055-1-AP (ITPRIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 26055-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26055-1-AP (ITPRIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HuH-7 using 26055-1-AP
IP result of anti-ITPRIP (IP:26055-1-AP, 4ug; Detection:26055-1-AP 1:800) with HuH-7 cells lysate 1200 ug.
IP experiment of A549 using 26055-1-AP
IP result of anti-ITPRIP (IP:26055-1-AP, 4ug; Detection:26055-1-AP 1:800) with A549 cells lysate 640 ug.

ITPRIP Polyclonal antibody

ITPRIP Polyclonal Antibody for WB, IP, ELISA

Cat No. 26055-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

bA127L20, DANGER, ITPRIP, KIAA1754, Protein DANGER

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, MDA-MB-453s cells
Positive IP detected in	A549 cells, HuH-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26055-1-AP targets ITPRIP in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22012 Product name: Recombinant human ITPRIP protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 201-547 aa of BC070108 Sequence: QVDRPLLCHLFVPFTPPEPYRFHPELWCSGRSVPLDRQGYGQIKVVRADGDTLSCICGKTKLGEDMLCLLHGRNSMAPPCGDMENLLCATDSLYLDTMQVMKWFQTALTRAWKGIAHKYEFDLAFGQLDSPGSLKIKFRSGKFMPFNLIPVIQCDDSDLYFVSHLPREPSEGTPASSTDWLLSFAVYERHFLRTTLKALPEGACHLSCLQIASFLLSKQSRLTGPSGLSSYHLKTALLHLLLLRQAADWKAGQLDARLHELLCFLEKSLLQKKLHHFFIGNRKVPEAMGLPEAVLRAEPLNLFRPFVLQRSLYRKTLDSFYEMLKNAPALISEYSLHVPSDQPTPKS 相同性解析による交差性が予測される生物種
Full Name	inositol 1,4,5-triphosphate receptor interacting protein
Calculated molecular weight	547 aa, 62 kDa
Observed molecular weight	62 kDa
GenBank accession number	BC070108
Gene Symbol	ITPRIP
Gene ID (NCBI)	85450
RRID	AB_2880354
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IWB1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IP protocol for ITPRIP antibody 26055-1-AP	Download protocol
WB protocol for ITPRIP antibody 26055-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

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