Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | mouse colon tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
20729-1-AP targets ITPR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | inositol 1,4,5-triphosphate receptor, type 3 |
| Calculated molecular weight | 304 kDa |
| Observed molecular weight | 250-300 kDa |
| GenBank accession number | NM_002224 |
| Gene Symbol | ITPR3 |
| Gene ID (NCBI) | 3710 |
| RRID | AB_3669343 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14573 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ITPRs family have three members: ITPR1, ITPR2 and ITPR3. For ITPR3, it is associated with cervical squamous cell carcinoma, glioblastoma, cholangiocarcinoma and other cancers. Inositol 1,4, 5-triphosphate receptor type 3 (ITPR3) was found to be apical-located in bile duct cells, and disruption of lipid raft in the segregated bile duct unit (IBDU) redistributes ITPR3 and impairs Ca2+ waves. (PMID: 35580861, PMID:35852334)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ITPR3 antibody 20729-1-AP | Download protocol |
| IHC protocol for ITPR3 antibody 20729-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Zhizichi decoction alleviates depressive-like behaviors through modulating mitochondria-associated membrane via the IP3R3-GRP75-VDAC1 complex | ||
Mol Cell Endocrinol High glucose elevates intracellular calcium level and induces ferroptosis in glomerular endothelial cells through the miR-223-3p/ITPR3 pathway |