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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ITPR1-specific Recombinant antibody (85101-1-RR)

    WB analysis using 85101-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85101-1-RR (ITPR1-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ITPR1-Specific Recombinant antibody (85101-1-RR)

    IHC staining of mouse brain using 85101-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85101-1-RR (ITPR1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ITPR1-Specific Recombinant antibody (85101-1-RR)

    IHC staining of mouse brain using 85101-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85101-1-RR (ITPR1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ITPR1-Specific Recombinant antibody

ITPR1-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85101-1-RR
Clone No.242274B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ITPR1, 242274B10, ACV, Inositol 1,4,5 trisphosphate receptor, Inositol 1,4,5-trisphosphate receptor type 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells, L02 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85101-1-RR targets ITPR1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name inositol 1,4,5-triphosphate receptor, type 1
Calculated molecular weight 314 kDa
Observed molecular weight 290-300 kDa
GenBank accession numberNM_001099952
Gene Symbol ITPR1
Gene ID (NCBI) 3708
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ14643
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

Protocols

Product Specific Protocols
WB protocol for ITPR1-Specific antibody 85101-1-RRDownload protocol
IHC protocol for ITPR1-Specific antibody 85101-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols