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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    WB analysis of mouse brain using 19962-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19962-1-AP (ITPR1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ITPR1-specific Polyclonal antibody (19962-1-AP)

    WB analysis using 19962-1-AP

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 19962-1-AP (ITPR1-specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    WB analysis of mouse liver using 19962-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19962-1-AP (ITPR1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    IP experiment of mouse brain using 19962-1-AP

    IP result of anti-ITPR1-specific (IP:19962-1-AP, 4ug; Detection:19962-1-AP 1:300) with mouse brain tissue lysate 3160 ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    IHC staining of human brain using 19962-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    IHC staining of human brain using 19962-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    IHC staining of human testis using 19962-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    IHC staining of human testis using 19962-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    IHC staining of mouse brain using 19962-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    IHC staining of mouse brain using 19962-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    IF Staining of mouse brain using 19962-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using ITPR1-specific antibody (19962-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ITPR1-specific Polyclonal antibody (19962-1-AP)

    IF Staining of mouse brain using 19962-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using ITPR1-specific antibody (19962-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using ITPR1-specific Polyclonal antibody (19962-1-AP)

    FC experiment of HepG2 using 19962-1-AP

    1X10^6 HepG2 cells were stained with 0.2 ug ITPR1-specific antibody (19962-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

ITPR1-specific Polyclonal antibody

ITPR1-specific Polyclonal Antibody for WB, IHC, IF-Fro, FC (Intra), IP, ELISA
Cat No. 19962-1-AP
Publications(49)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF-Fro, FC (Intra), IP, CoIP, ELISA

Synonyms

ITPR1, ACV, Inositol 1,4,5 trisphosphate receptor, Inositol 1,4,5-trisphosphate receptor type 1, INSP3R1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue, mouse liver tissue, L02 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected inmouse brain tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19962-1-AP targets ITPR1-specific in WB, IHC, IF-Fro, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, bovine, sheep, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name inositol 1,4,5-triphosphate receptor, type 1
Calculated molecular weight 314 kDa
Observed molecular weight 290-300 kDa
GenBank accession numberNM_001099952
Gene Symbol ITPR1
Gene ID (NCBI) 3708
RRIDAB_10638786
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14643
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

Protocols

Product Specific Protocols
FC protocol for ITPR1-specific antibody 19962-1-APDownload protocol
IF protocol for ITPR1-specific antibody 19962-1-APDownload protocol
IHC protocol for ITPR1-specific antibody 19962-1-APDownload protocol
IP protocol for ITPR1-specific antibody 19962-1-APDownload protocol
WB protocol for ITPR1-specific antibody 19962-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Commun

OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools

Authors - Ara Lee
View Article

Nat Commun

ER calcium depletion as a key driver for impaired ER-to-mitochondria calcium transfer and mitochondrial dysfunction in Wolfram syndrome

Authors - Mailis Liiv
View Article
mouseIF

Cell Discov

Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction.

Authors - Yi-yang Zhao
View Article
human

Neuron

Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7.

Authors - Colleen A Stoyas
View Article
humanWB

J Exp Clin Cancer Res

ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma.

Authors - Mengzhao Zhang
View Article
humanWB

Cancer Lett

MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.

Authors - Qi Sun
View Article
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