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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ITPKC Polyclonal antibody (14267-1-AP)

    WB analysis using 14267-1-AP (same clone as 14267-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using ITPKC Polyclonal antibody (14267-1-AP)

    WB analysis of human heart using 14267-1-AP (same clone as 14267-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ITPKC Polyclonal antibody (14267-1-AP)

    IP experiment of HepG2 using 14267-1-AP (same clone as 14267-1-PBS)

    IP result of anti-ITPKC (IP:14267-1-AP, 4ug; Detection:14267-1-AP 1:400) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human breast cancer using 14267-1-AP (same clone as 14267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human breast cancer using 14267-1-AP (same clone as 14267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human lymphoma using 14267-1-AP (same clone as 14267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human lymphoma using 14267-1-AP (same clone as 14267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human heart using 14267-1-AP (same clone as 14267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human heart using 14267-1-AP (same clone as 14267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ITPKC Polyclonal antibody (14267-1-AP)

    IF Staining of HepG2 using 14267-1-AP (same clone as 14267-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using ITPKC antibody 14267-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

ITPKC Polyclonal antibody, PBS Only

ITPKC Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14267-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14267-1-PBS targets ITPKC in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5583

Product name: Recombinant human ITPKC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-358 aa of BC060788

Sequence: MRRCPCRGSLNEAEAGALPAAARMGLEAPRGGRRRQPGQQRPGPGAGAPAGRPEGGGPWARTEGSSLHSEPERAGLGPAPGTESPQAEFWTDGQTEPAAAGLGVETERPKQKTEPDRSSLRTHLEWSWSELETTCLWTETGTDGLWTDPHRSDLQFQPEEASPWTQPGVHGPWTELETHGSQTQPERVKSWADNLWTHQNSSSLQTHPEGACPSKEPSADGSWKELYTDGSRTQQDIEGPWTEPYTDGSQKKQDTEAARKQPGTGGFQIQQDTDGSWTQPSTDGSQTAPGTDCLLGEPEDGPLEEPEPGELLTHLYSHLKCSPLCPVPRLIITPETPEPEAQPVGPPSRVEGGSGGFS

相同性解析による交差性が予測される生物種
Full Name inositol 1,4,5-trisphosphate 3-kinase C
Calculated molecular weight 75 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC060788
Gene Symbol ITPKC
Gene ID (NCBI) 80271
RRIDAB_2280728
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DU7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ITPKC(Inositol-trisphosphate 3-kinase C) is also named as IP3KC and belongs to the inositol phosphokinase (IPK) family. It can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate and is relatively weakly activated by the calcium-calmodulin complex. ITPKC also acts as a negative regulator of T-cell activation through the Ca(2+)/NFAT signaling pathway, and the C allele may contribute to immune hyperreactivity in Kawasaki disease(PMID:17160344).

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