Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
WB analysis of mouse thymus using 12816-1-AP (same clone as 12816-1-PBS)
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 12816-1-AP (same clone as 12816-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 12816-1-AP (same clone as 12816-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 12816-1-AP (same clone as 12816-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 12816-1-AP (same clone as 12816-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 12816-1-AP (same clone as 12816-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12816-1-AP (ITPKB Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 12816-1-AP (same clone as 12816-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 12816-1-AP (same clone as 12816-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

ITPKB Polyclonal antibody, PBS Only

ITPKB Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12816-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12816-1-PBS targets ITPKB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3456 Product name: Recombinant human ITPKB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC015009 Sequence: MAVYCYALNSLVIMNSANEMKSGGGPGPSGSETPPPPRRAVLSPGSVFSPGRGASFLFPPAESLSPEEPRSPGGWRSGRRRLNSSSGSGSGSSGSSVSSPSWAGRLRGDRQQVVAAGTLSPPGPEEAKRKLRILQRELQNVQVNQKVGMFEAHIQAQSSAIQAPRSPRLGRAHSPSPCPFRSSSQPPGRVLVQGARSEERRTKSWGEQCPETSGTDSGRKGGPSLCSSQVKKGMPPLPGRAAPTGSEAQGPSAFVRMEKGIPASPRCGSPTAMEIDKRGSPTPGTRSCLAPSLGLFGASLTMATEVAARVTSTGPHRPQDLALTEPSGRARELEDLQPPEALVERQGQFL 相同性解析による交差性が予測される生物種
Full Name	inositol 1,4,5-trisphosphate 3-kinase B
Calculated molecular weight	946 aa, 102 kDa
Observed molecular weight	102-110 kDa
GenBank accession number	BC015009
Gene Symbol	ITPKB
Gene ID (NCBI)	3707
RRID	AB_2129846
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P27987
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Inositol 1,4,5-trisphosphate 3-kinase isoform B (IITPKB) is also named as IP3KB and possesses important roles in the development of immune cells. IP3KB can be targeted to multiple cellular compartments, among them nuclear localization and binding in close proximity to the plasma membrane. The B isoform is the only IP3K that is almost ubiquitously expressed in mammalian cells(PMID:21148483).Three isoforms of IP3KB occur in mammals, which are distinguished by different molecular masses, sensitivity to Ca2+/CaM(calmodulin), intracellular distribution and tissue expression (PMID:16173920). ITPKB can be cleaved into many fragments, this antibody can recognize the N-terminal peptide fragment.(PMID:15130091)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

WB analysis of mouse thymus using 12816-1-AP (same clone as 12816-1-PBS)

WB analysis of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

WB analysis of mouse lung using 12816-1-AP (same clone as 12816-1-PBS)

WB analysis of mouse lung using 12816-1-AP (same clone as 12816-1-PBS)

WB analysis of rat brain using 12816-1-AP (same clone as 12816-1-PBS)

WB analysis of K-562 using 12816-1-AP (same clone as 12816-1-PBS)

IHC staining of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

IHC staining of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

IHC staining of human cervical cancer using 12816-1-AP (same clone as 12816-1-PBS)

IHC staining of human lung using 12816-1-AP (same clone as 12816-1-PBS)