Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
14407-1-AP targets ITM2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5692 Product name: Recombinant human ITM2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-263 aa of BC040437 Sequence: MVKIAFNTPTAVQKEEARQDVEALLSRTVRTQILTGKELRVATQEKEGSSGRCMLTLLGLSFILAGLIVGGACIYKYFMPKSTIYRGEMCFFDSEDPANSLRGGEPNFLPVTEEADIREDDNIAIIDVPVPSFSDSDPAAIIHDFEKGMTAYLDLLLGNCYLMPLNTSIVMPPKNLVELFGKLASGRYLPQTYVVREDLVAVEEIRDVSNLGIFIYQLCNNRKSFRLRRRDLLLGFNKRAIDKCWKIRHFPNEFIVETKICQE 相同性解析による交差性が予測される生物種 |
| Full Name | integral membrane protein 2A |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC040437 |
| Gene Symbol | ITM2A |
| Gene ID (NCBI) | 9452 |
| RRID | AB_2129683 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integral membrane protein 2A (ITM2A) is a single-pass type II membrane protein belonging to a family,which is composed of at least two other members ITM2B and ITM2C. ITM2A can be induced during thymocyte selection and T cell activation. In early stages of differentiation ITM2A may be associated with an inhibition of the initiation of chondrogenesis.Catalog#14407-1-AP recognises two band of 30 kDa and 45-48 kDa, and the additional 45-48 kDa band may be due to glycosylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ITM2A antibody 14407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adipocyte Itm2a silencing rescues lamin A mediated inhibition of 3T3-L1 adipocyte differentiation.
| ||
Front Immunol Integrating machine learning, bioinformatics and experimental verification to identify a novel prognostic marker associated with tumor immune microenvironment in head and neck squamous carcinoma |