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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    WB analysis using 21738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    WB analysis of A431 using 21738-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    WB analysis of A549 using 21738-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IP experiment of HeLa using 21738-1-AP

    IP result of anti-Integrin Beta 4 (IP:21738-1-AP, 4ug; Detection:21738-1-AP 1:500) with HeLa cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of mouse colon using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of mouse colon using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human placenta using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human placenta using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human pancreas cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human pancreas cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human breast cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human breast cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human pancreas cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human pancreas cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IF Staining of A431 using 21738-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 4 antibody (21738-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

Integrin beta 4 Polyclonal antibody

Integrin beta 4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21738-1-AP
Publications(39)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ITGB4, CD104, GP150, Integrin beta-4, integrin, beta 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, A431 cells, A549 cells, HT-29 cells, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse colon tissue, human placenta tissue, human pancreas cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21738-1-AP targets Integrin beta 4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16450

Product name: Recombinant human Integrin beta-4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-354 aa of BC126411

Sequence: ANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRRCNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFELEVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYRTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSN

相同性解析による交差性が予測される生物種
Full Name integrin, beta 4
Calculated molecular weight 1752 aa, 195 kDa
Observed molecular weight 200-240 kDa
GenBank accession numberBC126411
Gene Symbol Integrin beta 4
Gene ID (NCBI) 3691
RRIDAB_10733888
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP16144
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Protocols

Product Specific Protocols
IF protocol for Integrin beta 4 antibody 21738-1-APDownload protocol
IHC protocol for Integrin beta 4 antibody 21738-1-APDownload protocol
IP protocol for Integrin beta 4 antibody 21738-1-APDownload protocol
WB protocol for Integrin beta 4 antibody 21738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Host Microbe

Peptostreptococcus stomatis promotes colonic tumorigenesis and receptor tyrosine kinase inhibitor resistance by activating ERBB2-MAPK

Authors - Pingmei Huang
  • KD Validated
View Article
mouse,humanWB

Nat Commun

Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis.

Authors - Haoning Peng
View Article
humanWB,IHC

J Exp Clin Cancer Res

Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.

Authors - Binbin Ma
  • KD Validated
View Article

MedComm (2020)

Nicotinamide N-Methyltransferase Epigenetically Activates Fibronectin 1 Through H3K9me3 Remodeling in Clear Cell Renal Cell Carcinoma.

Authors - Lingling Wang
View Article
humanWB

Cell Death Dis

SOAT1 regulates cholesterol metabolism to induce EMT in hepatocellular carcinoma

Authors - Rongrong Fu
View Article
mouse,humanWB,IHC

Cell Commun Signal

NEDD4L mediates ITGB4 ubiquitination and degradation to suppress esophageal carcinoma progression

Authors - Yijun Shi
View Article
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