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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using ITGB1BP2 Polyclonal antibody (21359-1-AP)

    WB analysis of human heart using 21359-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21359-1-AP (ITGB1BP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ITGB1BP2 Polyclonal antibody (21359-1-AP)

    WB analysis of human heart using 21359-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21359-1-AP (ITGB1BP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ITGB1BP2 Polyclonal antibody (21359-1-AP)

    IF Staining of HepG2 using 21359-1-AP

    Immunofluorescent analysis of HepG2 cells, using ITGB1BP2 antibody 21359-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

ITGB1BP2 Polyclonal antibody

ITGB1BP2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21359-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MSTP015, Melusin, ITGB1BP, Integrin beta-1-binding protein 2, CHORDC3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman heart tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21359-1-AP targets ITGB1BP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15808

Product name: Recombinant human ITGB1BP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-329 aa of BC108901

Sequence: TVDFSEFLNIKGCTMGPHCAEKLPEAPQPEGPATSSSLQEQKPLNVIPKSAETLRRERPKSELPLKLLPLNISQALEMALEQKELDQEPGAGLDSLIRTGSSCQNPGCDAVYQGPESDATPCTYHPGAPRFHEGMKSWSCCGIQTLDFGAFLAQPGCRVGRHDWGKQLPASCRHDWHQTDSLVVVTVYGQIPLPAFNWVKASQTELHVHIVFDGNRVFQAQMKLWGVINVEQSSVFLMPSRVEISLVKADPGSWAQLEHPDALAKKARAGVVLEMDEEESDDSDDDLSWTEEEEEEEAMGE

相同性解析による交差性が予測される生物種
Full Name integrin beta 1 binding protein (melusin) 2
Calculated molecular weight 347 aa, 38 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC108901
Gene Symbol ITGB1BP2
Gene ID (NCBI) 26548
RRIDAB_10734427
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ITGB1BP2 antibody 21359-1-APDownload protocol
IF protocol for ITGB1BP2 antibody 21359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols