Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 27224-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 27224-1-AP
WB result of Integrin alpha-5 antibody (27224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin alpha-5 transfected HeLa cells.
WB analysis of HAP1 using 27224-1-AP
WB result of Integrin alpha-5/CD49e antibody (27224-1-AP; 1:20000; room temperature for 1.5 hours) with wild-type and Integrin alpha-5/CD49e knockout HAP1 cells.
WB analysis of human placenta using 27224-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 27224-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 27224-1-AP (ITGA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 27224-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of human placenta using 27224-1-AP
IP result of anti-Integrin Alpha 5 (IP:27224-1-AP, 4ug; Detection:27224-1-AP 1:1000) with human placenta tissue lysate 2600 ug.
IP experiment of human placenta using 27224-1-AP
IP result of anti-Integrin Alpha 5 (IP:27224-1-AP, 4ug; Detection:27224-1-AP 1:1000) with human placenta tissue lysate 4000 ug.
IHC staining of human placenta using 27224-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human placenta using 27224-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

Integrin alpha-5/CD49e Polyclonal antibody

Integrin alpha-5/CD49e Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 27224-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

Integrin Alpha-5, ITGA5, CD49 antigen-like family member E, CD49e, FNRA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HAP1 cells, HeLa cells, human placenta tissue, U2OS cells
Positive IP detected in	human placenta tissue
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below

Product Information

27224-1-AP targets Integrin alpha-5/CD49e in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26098 Product name: Recombinant human ITGA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 874-995 aa of NM_002205 Sequence: PINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSY 相同性解析による交差性が予測される生物種
Full Name	integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Calculated molecular weight	115 kDa
Observed molecular weight	135-150 kDa
GenBank accession number	NM_002205
Gene Symbol	Integrin alpha 5
Gene ID (NCBI)	3678
RRID	AB_2880807
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08648
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Integrin alpha-5/CD49e antibody 27224-1-AP	Download protocol
IHC protocol for Integrin alpha-5/CD49e antibody 27224-1-AP	Download protocol
IP protocol for Integrin alpha-5/CD49e antibody 27224-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	J Clin Transl Hepatol A Prognostic Nomogram for Hepatocellular Carcinoma Based on Wound Healing and Immune Checkpoint Genes Authors - Beiyuan Hu View Article
human	WB	Mol Cancer ZNF460-mediated circRPPH1 promotes TNBC progression through ITGA5-induced FAK/PI3K/AKT activation in a ceRNA manner Authors - Chuanpeng Zhang View Article
human	WB	Biomater Res Targeting Reprogrammed Cancer-Associated Fibroblasts with Engineered Mesenchymal Stem Cell Extracellular Vesicles for Pancreatic Cancer Treatment Authors - Pengcheng Zhou KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 27224-1-AP

WB analysis of HeLa using 27224-1-AP

WB analysis of HAP1 using 27224-1-AP

WB analysis of human placenta using 27224-1-AP

WB analysis of A549 using 27224-1-AP