Various lysates were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

K-562 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

U-251 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IP result of anti-ITCH (IP:20920-1-AP, 4ug; Detection:20920-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

HeLa lysates prepared and IP of ITCH performed using 2.0 μg of 20920-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20920-1-AP (ITCH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20920-1-AP (ITCH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).