Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse thymus tissue, mouse spleen tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87417-1-RR targets CD103 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg10465 Product name: Recombinant mouse ITAE protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-Twin-Strep-Tag Sequence: RPVVDLTVSMTFTPDALPMVFIGKMDVKLCFEVDSSGVASEPGLREMFLNFTVDVDVTKQRQRLQCEDSSGCQSCLRKWNGGSFLCEHFWLISTEELCEEDCFSNITIKVTYEFQTSGGRRDYPNPTLDHYKEPSAIFQLPYEKDCKNKVFCIAEIQLTTNISQQELVVGVTKEVTMNISLTNSGEDSYMTNMALNYPRNLQFKKIQKPVSPDVQCDDPKPVASVLVMNCKIGHPILKRSSVNVSVTWQLEESVFPNRTADITVTISNSNEKSLARETRSLQFRHAFIAVLSRPSVMYMNTSQSPSDHKEFFFNVHGENLFGAVFQLQICVPIKLQDFQIVRVKNLTKTQDHTECTQSQEPACGSDPVQHVKEWHSVVCAITSNKENVTVAAEISVGHTKQLLRDVSELPILGEISFNKSLYEGLNAENHRTKITVIFLKEEETRS 相同性解析による交差性が予測される生物種 |
| Full Name | integrin alpha E, epithelial-associated |
| Observed molecular weight | 160 kDa |
| Gene Symbol | CD103 |
| Gene ID (NCBI) | 16407 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD103 antibody 87417-1-RR | Download protocol |
| WB protocol for CD103 antibody 87417-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |