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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ISOC1 Polyclonal antibody

ISOC1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25844-1-AP

Synonyms

CGI 111, ISOC1

Validation Data Gallery

Applications:
  • WB analysis using 25844-1-AP

    WB analysis using 25844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25844-1-AP (ISOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 25844-1-AP

    WB analysis using 25844-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25844-1-AP (ISOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 25844-1-AP

    IP experiment of mouse liver using 25844-1-AP

    IP result of anti-ISOC1 (IP:25844-1-AP, 4ug; Detection:25844-1-AP 1:300) with mouse liver tissue lysate 4000ug.

Tested Applications

Positive WB detected inHT-29 cells, mouse liver tissue, HepG2 cells, SH-SY5Y cells, mouse embryo tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25844-1-AP is ISOC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISOC1 fusion protein Ag22611 相同性解析による交差性が予測される生物種
Full Name isochorismatase domain containing 1
Observed molecular weight 32 kDa
GenBank accession numberBC014105
Gene symbol ISOC1
Gene ID (NCBI) 51015
RRIDAB_2918095
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ISOC1 antibody 25844-1-APDownload protocol
IP protocol for ISOC1 antibody 25844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols