Validation Data Gallery
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Tested Applications
| Positive WB detected in | HT-29 cells, TT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30152-1-AP targets ISM1 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ISM1 fusion protein Ag32761 相同性解析による交差性が予測される生物種 |
| Full Name | isthmin 1 homolog (zebrafish) |
| Calculated molecular weight | 52kd |
| Observed molecular weight | 52 kDa, 70 kDa |
| GenBank accession number | NM_080826 |
| Gene Symbol | ISM1 |
| Gene ID (NCBI) | 140862 |
| RRID | AB_3669703 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | B1AKI9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ISM1, also known as C20orf82. It is expected to be located in extracellular space, and the protein is enriched in the thyroid gland tissue. Acts as an angiogenesis inhibitor. The protein is predicted to be involved in negative regulation of angiogenesis. Since the molecular weight of the detected ISM1 was larger than expected (52 kD), ISM1 is likely subject to posttranslational modifications. Glycosylation is commonly found in many secreted proteins, and analysis of the ISM1 protein sequence predicted two putative N-glycosylation sites at positions N39 and N282. (PMID: 31171630)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ISM1 antibody 30152-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |