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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ISLR Recombinant monoclonal antibody (83782-5-RR)

    WB analysis using 83782-5-RR (same clone as 83782-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83782-5-RR (ISLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83782-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse retina tissue using ISLR Recombinant monoclonal antibody (83782-5-RR)

    WB analysis of mouse retina using 83782-5-RR (same clone as 83782-5-PBS)

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 83782-5-RR (ISLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83782-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using ISLR Recombinant monoclonal antibody (83782-5-RR)

    IHC staining of mouse eye using 83782-5-RR (same clone as 83782-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83782-5-RR (ISLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83782-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ISLR Recombinant monoclonal antibody (83782-5-RR)

    IF Staining of NIH/3T3 using 83782-5-RR (same clone as 83782-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ISLR antibody (83782-5-RR, Clone: 240727D4 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83782-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83782-5-PBS

    Affinity and Kinetic Characterization of 83782-5-PBS

    Biolayer interferometry (BLl) kinetic assays of 83782-5-PBS against Human ISLR were performed. The affinity constant is 1.30 nM.

  • Affinity and Kinetic Characterization of 83782-5-RR

    Affinity and Kinetic Characterization of 83782-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83782-5-RR against Human ISLR were performed. The affinity constant is 0.369 nM.

ISLR Recombinant monoclonal antibody, PBS Only

ISLR Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83782-5-PBS
Clone No.240727D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

240727D4, HsT17563, Immunoglobulin superfamily containing leucine-rich repeat protein, UNQ189/PRO215

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83782-5-PBS targets ISLR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19446

Product name: Recombinant human ISLR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 318-402 aa of BC022478

Sequence: GKLEEGTYSCLATNELGSAESSVDVALATPGEGGEDTLGRRFHGKAVEGKGCYTVDNEVQPSGPEDNVVIIYLSRAGNPEAAVAE

相同性解析による交差性が予測される生物種
Full Name immunoglobulin superfamily containing leucine-rich repeat
Calculated molecular weight 428 aa, 46 kDa
Observed molecular weight44-46 kDa
GenBank accession numberBC022478
Gene Symbol ISLR
Gene ID (NCBI) 3671
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO14498
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The Immunoglobulin superfamily containing leucine-rich repeat protein(ISLR), is involved in various biological events, such as embryonic development, Gaucher's disease, and replicative senescence of fibroblasts in some organs, including the heart, pancreas, and bone marrow. ISLR, also known as meflin, is a newly discovered marker of mesenchymal stem cells, which is involved in pathological fibrosis and the cancer microenvironment. (PMID: 34713300)

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