Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells |
Positive IP detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
24639-1-AP targets ISG20L2 in WB, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ISG20L2 fusion protein Ag20026 相同性解析による交差性が予測される生物種 |
Full Name | interferon stimulated exonuclease gene 20kDa-like 2 |
Calculated molecular weight | 353 aa, 39 kDa |
Observed molecular weight | 40 kDa |
GenBank accession number | BC000575 |
Gene Symbol | ISG20L2 |
Gene ID (NCBI) | 81875 |
RRID | AB_2879649 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H9L3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ISG20L2, also named HSD38, is a member of a vertebrate exonucleases family. ISG20L2 is composed of at least two functional domains, one at its N-terminal half allowing intracellular localization and association with the binding partners and the other at its C-terminal half bearing the 3` to 5` exoribonuclease activity. The molecular weight of ISG20L2 is 39 kDa (PMID: 18065403).
Protocols
Product Specific Protocols | |
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WB protocol for ISG20L2 antibody 24639-1-AP | Download protocol |
IP protocol for ISG20L2 antibody 24639-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
JCI Insight ISG20L2 suppresses bortezomib anti-myeloma activity by attenuating bortezomib binding to PSMB5 | ||
Nucleic Acids Res Molecular mechanism of human ISG20L2 for the ITS1 cleavage in the processing of 18S precursor ribosomal RNA |