Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | Raji cells, Daudi cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 16 publications below |
| IHC | See 5 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
22097-1-AP targets ISG20 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, canine, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17303 Product name: Recombinant human ISG20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC007922 Sequence: MAGSREVVAMDCEMVGLGPHRESGLARCSLVNVHGAVLYDKFIRPEGEITDYRTRVSGVTPQHMVGATPFAVARLEILQLLKGKLVVGHDLKHDFQALKEDMSGYTIYDTSTDRLLWREAKLDHCRRVSLRVLSERLLHKSIQNSLLGHS 相同性解析による交差性が予測される生物種 |
| Full Name | interferon stimulated exonuclease gene 20kDa |
| Calculated molecular weight | 181 aa, 20 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC007922 |
| Gene Symbol | ISG20 |
| Gene ID (NCBI) | 3669 |
| RRID | AB_2878994 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AZ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ISG20 antibody 22097-1-AP | Download protocol |
| WB protocol for ISG20 antibody 22097-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol Activation of sclerostin inhibits Isg20-Mediated aerobic glycolysis ameliorating renal Fibrosis: the renoprotective mechanism of hederagenin in CKD | ||
Mol Cell RNA exonuclease REXO4 resolves m(6)A-marked R-loops and suppresses anti-tumor immunity. | ||
Nucleic Acids Res Internal RNA 2'O-methylation in the HIV-1 genome counteracts ISG20 nuclease-mediated antiviral effect
| ||
Cell Rep Enzymolysis-based RNA pull-down identifies YTHDC2 as an inhibitor of antiviral innate response | ||
Cancer Cell Int Identification and validation of a novel glycolysis-related gene signature for predicting the prognosis in ovarian cancer. |