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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ISCA2 Polyclonal antibody

ISCA2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13200-1-AP

Synonyms

c14_5557, HBLD1, ISA2, ISCA2

Validation Data Gallery

Applications:
  • WB analysis using 13200-1-AP

    WB analysis using 13200-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13200-1-AP (ISCA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13200-1-AP

    IHC staining of human liver cancer using 13200-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13200-1-AP (ISCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 13200-1-AP

    IHC staining of human liver cancer using 13200-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13200-1-AP (ISCA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 13200-1-AP

    IHC staining of human breast cancer using 13200-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13200-1-AP (ISCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 13200-1-AP

    IHC staining of human breast cancer using 13200-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13200-1-AP (ISCA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 13200-1-AP

    IF Staining of A431 using 13200-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 13200-1-AP (ISCA2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13200-1-AP targets ISCA2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISCA2 fusion protein Ag3941 相同性解析による交差性が予測される生物種
Full Name iron-sulfur cluster assembly 2 homolog (S. cerevisiae)
Calculated molecular weight 154 aa, 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC032893
Gene symbol ISCA2
Gene ID (NCBI) 122961
RRIDAB_2877924
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ISCA2 antibody 13200-1-APDownload protocol
IHC protocol for ISCA2 antibody 13200-1-APDownload protocol
IF protocol for ISCA2 antibody 13200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Sci Rep

Systematic analysis of expression profiles and prognostic significance for MMDS-related iron–sulfur proteins in renal clear cell carcinoma

Authors - Ling Yang
View Article
mouseWB

Neurosci Bull

Intermittent Theta Burst Stimulation Attenuates Cognitive Deficits and Alzheimer's Disease-Type Pathologies via ISCA1-Mediated Mitochondrial Modulation in APP/PS1 Mice

Authors - Yang Zhu
View Article